FIG. 3.

Lysosome and Golgi apparatus localization in J774.2 cells. Cells were incubated for 1 h with PGA. PGA bound to the cell exterior was blocked by incubation with unlabeled anti-PGA MAb. The Golgi apparatus was localized by coincubation with Alexa Fluor 555-labeled cholera toxin subunit B (top panel, red). The cells were fixed and permeabilized, and the lysosomes were stained with rat LAMP-1 and Alexa Fluor 555-labeled goat anti-rat IgG (bottom panel, red). PGA in the cell interior was stained with Alexa Fluor 488-labeled F26G3 (green). The results are representative of three experiments with similar results.