TABLE 2.

Equilibrium constants determined by flow cytometry for HlgB* binding in the presence of LukS-PV or HlgC on human PMNs, monocytes, and lymphocytes^a

Cells	HlgB* plus LukS-PV		HlgB* plus HlgC
	Kd (nM)	Maximal binding (SFUB)	Kd (nM)	Maximal binding (SFUB)
PMNs	1.1 ± 0.2b	484 ± 47b	0.37 ± 0.11b	474 ± 53b
Monocytes	0.84 ± 0.31b	143 ± 22b	0.11 ± 0.02c	200 ± 10c
Lymphocytes	NBd	NB	3.6 ± 1.0b	132 ± 12b

^aAll data were obtained as described in the legend to Fig. 1. The HlgC concentrations used were 2 nM for PMNs and monocytes and 50 nM for lymphocytes. The data are means ± standard deviations. SFU_B, standardized fluorescent units for HlgB*.

^bn = 4.

^cn =3.

^dNB, no LukS-PV binding.