FIG. 2.

Intracellular growth kinetics of the pmrA and pmrB mutants of L. pneumophila within protozoa. The intracellular growth kinetics of the pmrA and pmrB mutant in A. polyphaga (A) and T. pyriformis (B) were determined. The infection was carried out in triplicates for 1 h, followed by 1 h of gentamicin treatment to kill extracellular bacteria in case of A. polyphaga. The infected monolayers were lysed at different time intervals and plated onto agar plates for colony enumeration. The experiment was done three times, and the data are representative of one independent experiment. Error bars represent standard deviations, but some were too small to appear in the figure.