FIG. 5.

in cis rescue of intracellular growth of the pmrB mutant within communal phagosomes harboring the WT strain in hMDMs. The hMDMs were simultaneously infected with the GFP-positive L. pneumophila strain AA100 (WT) and one of the mutants—dotA, htrA, or pmrB—followed by fixation at 10 h after infection (see Materials and Methods). Macrophages harboring phagosomes containing both strains (GFP-WT and the mutants) were scored. Representative confocal images and quantitation are shown in panels A and C, respectively. Panel B shows the replication status of single infection by the WT strain and the pmrB mutant at 10 h postinfection. The results are representative of three independent experiments performed in triplicates. Error bars represent the standard deviation.