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. 2008 Oct 20;77(1):341–347. doi: 10.1128/IAI.01097-08

FIG. 4.

FIG. 4.

Host cell damage promotes ETEC adherence. (A) Immunoblot of IPEC-J2 cells after 1 h of treatment with 100 ng/ml LT or after 4 h of infection with E. coli G58-1 or wt or ΔeltAB ETEC at an MOI of 10. Blots were probed with rabbit polyclonal antisera against active caspase 3. (B) ETEC adherence (mean CFU/ml ± SD) following treatment of IPEC-J2 cells with 100 μM camptothecin (campt), with or without 100 μM Ac-DEVD-CHO, for 1 h prior to ETEC infection. (C) ETEC adherence (mean CFU/ml ± SD) to naïve host cells following prestimulation of bacterial inocula with sterile cell-free supernatants derived from donor cells treated with camptothecin, with or without Ac-DEVD-CHO. (D) Relative expression of K88ac-CAT in the presence of supernatants derived from host cells treated with camptothecin, with or without Ac-DEVD-CHO. Data are plotted as relative CAT activities versus that of the bacterial culture additive.