FIG. 1.

Kinetics of the humoral immune response elicited after immunizations with L-Omp16 (A) or L-Omp19 (B). BALB/c mice were immunized with 10 μg (▵) or 30 μg (▪) of L-Omp16 or L-Omp19 protein in IFA i.p. at days 0 and 15, and bled retro-orbitally at the indicated days after the first immunization. Anti-Omp16 or anti-Omp19 IgG titers were determined by ELISA. Each point represents the mean Ab titer from 5 mice in each group ± standard error of the mean. Data are representative of two separate experiments. Star, significantly different from the 10-μg immunized group (P < 0.05).