FIG. 3.
IFN-γ production in spleen cells from mice immunized with PBS or with U-Omp16, or U-Omp19 protein plus IFA. Spleen cells were cultured at 4 × 106 cells/ml with HS extract (20 μg/ml), ConA (5 μg/ml), or complete medium alone (−) for 72 h. Each sample was assayed in duplicate wells. IFN-γ in these culture supernatants was measured by sandwich ELISA. Data represent the mean ± standard error of the mean from each group of five mice. Data are representative of two separate experiments. Star, significantly different from the same stimulus in PBS-immunized mice (P < 0.05).