TABLE 3.

Contribution of CD4⁺ and CD8⁺ T lymphocytes, induced by immunization with U-Omp16 or U-Omp19 protein in IFA, to protection against B. abortus 544 in BALB/c mice

Vaccine	Treatment	Log10 CFU of B. abortus 544a	Protection (U)
B. abortus S19	None	3.86 ± 0.46b	2.00
U-Omp16+IFA	IgG	4.48 ± 0.55b	1.38
U-Omp16+IFA	Anti-CD4+	5.20 ± 0.51c	0.66
U-Omp16+IFA	Anti-CD8+	5.07 ± 0.46c	0.79
U-Omp19+IFA	IgG	4.28 ± 0.48b	1.58
U-Omp19+IFA	Anti-CD4+	5.18 ± 0.59c	0.68
U-Omp19+IFA	Anti-CD8+	5.21 ± 0.54c	0.65
PBS+IFA	None	5.86 ± 0.06c	0

^aThe content of bacteria in spleens is represented as the mean log CFU ± standard deviation per group (n = 5 mice per group).

^bSignificantly different from PBS-immunized mice (P < 0.01 estimated by Dunnett's test).

^cSignificantly different from S19-immunized mice (P < 0.01 estimated by Dunnett's test).