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IFN-γ secretion by lymphocytes from different groups of immunized mice on restimulation with M. w Ag or M. tuberculosis (M.tb) Ag in vitro. Sonicate supernatant of M. w or M. tuberculosis at a concentration of 2 μg per well was used as the Ag for in vitro restimulation. Data are expressed as the mean ± SEM (n = 4 mice per group). ***, P < 0.001.
