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Cell-mediated cytotoxicity of CD8+ T cells isolated from different groups of immunized or unimmunized mice toward M. tuberculosis Ag-pulsed syngeneic macrophages from BAL fluid. Target cells were labeled with PKH-67. The nuclear marker 7-aminoactinomycin D was added to distinguish between live and dead cells. Cells were taken from four mice per group for this study. The results shown here are representative of two similar experiments. *, P < 0.05; ***, P < 0.001.
