FIG. 3.
Absence of CSF-1 results in decidual tissue destruction and fetal loss after L. monocytogenes challenge during early gestation. (A) Representative photographs of GD8 pregnant uteri (U) either uninfected (iii [Csf1op/op]) or challenged with 104 CFU of L. monocytogenes i.v. on GD5 and harvested at 72 h p.i. (i [Csf1op/op] and ii [Csf1op/+]). * marks an example of an implantation site, which is resorbed in the Csf1op/op uterus at 72 h p.i. (B) Pregnant mice (both Csf1op/op and Csf1op/+) were infected i.v. on GD5 or -6 with 104 CFU of L. monocytogenes. Mice were sacrificed on GD8 (at 48 or 72 h p.i.), and cross sections at the implantation sites were stained with hematoxylin and eosin. (Top) Csf1op/+, either uninfected (a) or 48 (b) and 72 (c) h p.i.; (bottom) Csf1op/op, either uninfected (d) or 48 (e) and 72 (f) h p.i. Note decidual tissue necrosis and leukocyte infiltration in the Csf1op/op animal (e) and resorption of the fetal tissue at 72 h p.i. (f). Bar = 100 μm.