FIG. 2.

Fine specificity of the humoral anti-LF response following A/J immunization. IgG antibody binding to decapeptides of LF overlapping by eight amino acids was measured using a solid-phase ELISA. The data are the antibody responses in sera from rLF-adjuvant-immunized mice on days 14, 28, and 42 and in sera from mice immunized with adjuvant alone. The data are the averages of the results of three independent immunization and mapping experiments. Mapping within each experiment was performed using sera pooled from 18 to 20 mice. Epitopes were numbered in order of reproducibility and reactivity at day 42, as shown in Table 1. The epitope numbers correspond to the epitope numbers in Tables 1 and 2. The horizontal lines indicate extended antigenic regions of epitopes 3 and 9 as defined at day 42.