Skip to main content
. 2008 Oct 27;77(1):539–548. doi: 10.1128/IAI.01034-08

FIG. 3.

FIG. 3.

Asc10 surface expression by the Asc10 mutant strains, as determined by Western blot analysis of purified cell wall fractions. All strains used in Western blot analyses had an OG1SSp background. OG1SSp(pCF10) produced mainly a 150-kDa band, which is larger than the predicted 137-kDa band for Asc10. This may have been due to glycosylation or association of LTA with Asc10. The electrophoretic patterns of the Asc10 derivatives, including the multiple banding patterns, have been observed previously (10, 33). The prgB deletion mutant OG1SSp(pCF10-8) was analyzed on a separate Western blot, and no Asc10 surface expression was detected. The smaller bands observed for pCF10-4, pCF10-5, and pCF10-6 were due to large deletions in the prgB gene.