FIG. 6.

Adherence of mutant Asc10 purified proteins to ECM proteins. The amounts of bound Asc10 proteins were measured by ELISA, and increasing absorbance represented larger amounts of Asc10 protein adhering to the ECM substrate. (A) Asc10 binding to fibrinogen. The N-terminal aggregation domain deletion variant protein (encoded by pCF10-4), but not the wild-type protein, was found to bind fibrinogen. (B) The N-terminal aggregation domain deletion (encoded by pCF10-4) and double aggregation domain (encoded by pCF10-5) variant proteins, but not the wild-type protein, were able to bind vitronectin. (C) The central aggregation domain insertion variant protein (encoded by pCF10-1), but not the wild-type protein, bound von Willebrand factor. The data in each graph are representative of the results of at least three experiments.