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Transmission by inoculation of salivary gland Anaplasma homogenates. Calves were inoculated with homogenates containing 108.4 St. Maries strain organisms (C31861 and C32003), 107.2 vaccine strain organisms (C1201 and C1205), or 108.2 vaccine strain organisms (C1210 and C1213). PCR amplification of msp5 from preinoculation blood (lane 1) or during acute bacteremia (lane 2) is shown. The positions of the molecular size markers are indicated on the right.
