FIG. 2.
Phosphorylation levels of seven proteins involved in cell survival responses are relatively unchanged during C. burnetii infection. Lysates of mock- and C. burnetii phase II-infected THP-1 cells were harvested at the indicated times postinfection and processed for the Bio-Plex assay as described in Materials and Methods. Protein phosphorylation levels of infected cell lysates (gray bars) were compared to those of uninfected cell lysates (black bars). Only p90RSK and Src showed slightly elevated phosphorylation at 24 to 72 hpi. p, phosphorylated. Samples were analyzed in triplicate, and results are representative of those found in two independent experiments. Error bars represent the standard deviation from the mean. An asterisk indicates a P value of <0.05 in comparison to uninfected control cells as determined by a Student's t test.