TABLE 1.

Primers used in this study

Primer	Use	Sequence (5′-3′)
Primers used for RT-PCR
GAPDH-F	RT-PCR GAPDH control	GGAATCAACGGTTTCGGTCG
GAPDH-R		CAATAAAAGGGTCGTTGA
ACT1-F	RT-PCR ACTIN control	AACATTGTCATGTCTGGTGGT
ACT1-R		CGGTGATCTCCTTTTGCATAC
ALL1-RT-for	RT-PCR of ALL1	CGTTACCCAAGGTGTCAAGGA
ALL1-RT-rev		CTTGAGGCCTGATATGCTCCT
Primers used for plasmid construction for homologous recombination
Neo-F	Amplification of neomycin cassette	CCATATGTTGGTAA AACGACGGCCAGTGAATTGTA
Neo-R		CCATGAATTGGCAGGAAACAGCTATGACCATGATT
ALL1-R-For	Amplification of ALL1 upstream nucleotides	CCATTTCTTGGTAACCTGTTGTTCAACGCGAGACTG
ALL1-R-Rev		CCATAAATTGGCTTCGACTTCCTTCCGGCTATATTCC
ALL1-L-For	Amplification of ALL1 downstream nucleotides	CCATAGATTGGAGGTTTGGGGTTGATTTCGAAGCT
ALL1-L-Rev		CCATCATTTGGCGAAAGTAATGTGATGGACCCAAGA
pUC19-F	Amplification of origin of replication and ampicillin resistance gene	CCATTTTTTGGGAAAGGGCCTCGTGATACGCCT
pUC19-R		CCATTCTTTGGGCTTTCCAGTCGGGAAACCTGT
ALL1-F	Complementation of ALL1	CTCGAGACGGGACCTACAGGTACATTCA
ALL1-R		TCTAGAAGAGCGTTTGGAAACACCAC
ALL1p-F	Recombinant ALL1 protein	ATGTCCGGCGTTACCCAAGGT
ALL1p-R		TTAAAGAGCCTGGGTCTTGCT