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Abi1 is tyrosine phosphorylated during infection in an Abl-dependent manner. HeLa cells were pretreated with 10 μM STI571 (STI571) prior to infection with strain SL1344 or VV341 for 30 min. Abi1 immunoprecipitates were immunoblotted (IB) for tyrosine phosphorylation using a combination of 4G10 and PY99 antibodies as described in Materials and Methods. IP, immunoprecipitation; IgG, immunoglobulin G.
