Biofilm development in flow cells. (A) Newman and derivatives: CYL6938, Newman(pLI50); CYL6969, Newman Δrbf(pLI50); CYL6975, Newman Δrbf(pYL8565). (B) UAMS1 and derivatives: CYL6939, UAMS-1(pLI50); CYL6970, UAMS-1 Δrbf(pLI50); CYL6976, UAMS-1 Δrbf(pYL8565). Selected pictures taken every 30 min are shown with the time point indicated above each image. The medium flow was from left to right.