TABLE 1.
S. aureus strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| 8325-4 | Prophage-free laboratory strain | J. Iandolo |
| RN4220 | 8325-4r− | 21 |
| Newman | Wild-type strain | T. Foster |
| UAMS-1 | S. aureus clinical isolate | 15 |
| CYL385 | 8325-4 Δspa rbf::Tn917 | 23 |
| CYL6938 | Newman(pLI50) | This study |
| CYL6932 | Newman(pYL8565) | This study |
| CYL6633 | Newman rbf::Tn917 | This study |
| CYL6962 | Newman Δrbf | This study |
| CYL6969 | Newman Δrbf(pLI50) | This study |
| CYL6975 | Newman Δrbf(pYL8565) | This study |
| CYL6933 | UAMS-1(pYL8565) | This study |
| CYL6965 | UAMS-1 Δrbf | This study |
| CYL6939 | UAMS-1(pLI50) | This study |
| CYL6970 | UAMS-1 Δrbf(pLI50) | This study |
| CYL6976 | UAMS-1 Δrbf(pYL8565) | This study |
| Plasmids | ||
| pGEM-T Easy | Cloning vector | Promega |
| pKOR1 | Vector for allele replacement | 1 |
| pYL8565 | 3.3-kb fragment containing rbf in pLI50 | 23 |