Skip to main content
. 2008 Oct 27;77(1):274–285. doi: 10.1128/IAI.00515-08

FIG. 3.

FIG. 3.

Growth and germination of B. anthracis wild-type and mutant strains. (A) Vegetative growth of the wild-type Sterne strain and SOD deletion mutants in BHI broth over time after inoculation with cultures grown overnight. (B) Vegetative outgrowth of the wild-type Sterne strain and SOD deletion mutants in modified G broth over time after inoculation with 5 × 108 heat-activated spores. (C) Kinetics of germination of spores from the parent and mutant strains. Spores were inoculated in 5% BHI broth and grown at 37°C and 225 rpm, and samples were heat treated to inactivate vegetative cells at the time points shown. Colony counts following heat treatment represent the numbers of heat-resistant spores. Percent germination at test points (tx) indicate the numbers of spores that germinated at each time point relative to the spore count at the starting point (t0). *, the levels of germination of Δsod15 ΔsodA1 and Δsod15 ΔsodA1 ΔsodC ΔsodA2 spores were statistically lower than those of the wild type at each time point (P < 0.001); **, the level of Res15,A1 germination was lower than that of the wild type at the 5-min (P < 0.001), 10-min (P = 0.002), and 30-min (P = 0.007) time points, but by 60 min, the differences were not significant (P = 0.10).