FIG. 5.

Quantitative comparison of spore-macrophage interactions between the B. anthracis parent strain, the MIGD101 mutant and the ΔgerHA mutant. Spores were incubated with RAW 264.7 macrophages at an MOI of 10. After a 12-h incubation, spores and vegetative B. anthracis were collected, and CFU were counted prior to and after a 70°C incubation for 45 min. The bar graph presents the total CFU and the heat-resistant CFU (n = 3). Error bars indicate ± the standard error of the mean, and the P values were <0.05.