TABLE 1.
MIGD clones identified during enrichment
| Gene(s)a | Description | Genotype | No. of clonesb (% of total) | B. anthracis strain |
|---|---|---|---|---|
| BAs2807/BAs2808 | Insertion between BAs2807 (449 bp at the 5′ side of major facilitator family transporter) and BAs2808 (298 bp at the 5′ side of glycosyltransferase, group 2 family protein) | 41 (36.28) | MIGD101 | |
| BAs4630 | Spore germination protein GerHA | gerHA::TnHimar1 | 24 (21.24) | MIGD102 |
| BAs4631 | Spore germination protein GerHB | gerHB::TnHimar1 | 10 (8.85) | MIGD103 |
| BAs4632 | Spore germination protein GerHC | gerHC::TnHimar1 | 2 (1.77) | MIGD104 |
| BAs0229 | Major facilitator transporter | BAs0229::TnHimar1 | 10 (8.85) | MIGD105 |
| BAs5014 | Hypothetical protein | BAs5014::TnHimar1 | 7 (6.19) | MIGD106 |
| BAs5265 | cGMP specific and stimulated phosphodiesterase/adenylate cyclase | BAs5265::TnHimar1 | 6 (5.31) | MIGD107 |
| BAs0177 | ABC transporter, substrate-binding protein, putative | BAs0177::TnHimar1 | 1 | MIGD108 |
| BAs0194 | Conserved domain protein | BAs0194::TnHimar1 | 1 | MIGD109 |
| BAs0437 | Phage transcriptional regulator, ArpU family | BAs0437::TnHimar1 | 1 | MIGD110 |
| BAs4927 | Conserved domain protein | BAs4927::TnHimar1 | 1 | MIGD111 |
| BAs5220 | Multidrug resistance protein, putative | BAs5220::TnHimar1 | 1 | MIGD112 |
| GBAA pXO1-0130/GBAA pXO11-0218 | Insertion between GBAA pXO1-0130 and GBAA pXO11-0218 | 3 (2.66) | MIGD113 | |
| GBAA pXO1-0122/GBAA pXO1-0123 | Insertion between GBAA pXO1-0122 and GBAA pXO1-0123 | 1 | MIGD114 | |
| GBAA pXO1-0064 | Conjugation protein, TraG/TraD family | pXO10064::TnHimar1 | 1 | MIGD115 |
| GBAA pXO1-0108 | Putative membrane protein | pXO10108::TnHimar1 | 1 | MIGD116 |
| GBAA pXO1-0164 | pagA, protective antigen | pXO10164::TnHimar1 | 1 | MIGD117 |
| GBAA pXO1-0224 | Conserved hypothetical protein | pXO10224::TnHimar1 | 1 | MIGD118 |
a
BLASTn analysis was performed using the genome of B. anthracis Sterne and the NCBI nonredundant GenBank database as necessary. In all cases, only significant hits (e value cutoff of <10−4) are listed.
b
That is, the number of candidate clones for which a nonambiguous sequence was obtained via RATE analysis.