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Disruption of the actin cytoskeleton does not affect binding of PA to ANTXR1-sv1 or ANTXR1-sv2. Receptor-negative cells or cells expressing high or low levels of ANTXR1-sv1 or ANTXR1-sv2 were treated with latrunculin A or DMSO vehicle at 37°C for 45 min and then incubated with 10−8 M PASSSR for 2 h at 4°C. Cells were washed with PBS and lysed, and the lysates were subjected to Western blotting with anti-PA antibody.
