TABLE 1.
Oligonucleotide primers used for construction of HVR deletion mutants, as well as for PCR and sequencing, in this study
| Primer ID | Primer sequence (5′ → 3′) |
|---|---|
| For construction of avian HEV HVR deletion mutants | |
| AvF | CCCGTTAACTGCGCACCACCGGCCG |
| Avf | CCCGTTAACGGCGGACCTGAGGTCA |
| AvR | CGGGTTAACAAGCCAGTCGGCGGCA |
| Avr | CGGGTTAACCTGTGGCGGCAAGGGC |
| For construction of swine HEV HVR-deletion mutants | |
| SwF | GACATCGCCGCTCGAGCCTCCCGCCTAA |
| Swf1 | ACATCTGGCTTTTCTCGCACTCGTCGTCTC |
| Swf2 | CCTGAGGCGGCCGCCCGTAAGCCACCAACA |
| Swf3 | CTGCCCCACCCTACCTTACCCAGCTCCATT |
| Swf4 | ACATCTGGCTTTTCTTTACCCAGCTCCATT |
| SwR | GGGACCTGGTATATACCCGAGCCTAGAA |
| Swr1 | AGAAAAGCCAGATGTTGACCAAGTCCGGGT |
| Swr2 | GGCGGCCGCCTCAGGGGGGGAGAAATCGCT |
| Swr3 | GGTAGGGTGGGGCAGCCCCGGGGCAGCGGC |
| For construction of genotype 1 human HEV replicon HVR-deletion mutants | |
| HuF | GGGAGCATGCTCAGAAGTTTATAACACGCC |
| Huf1 | TCGGAGGTTGATGCTCGCCGCCTGCTCTTT |
| Huf2 | CCCCCTGCACCGGATGGCGCTACCGCCGGG |
| HuR | GTACCTCTGGTAAAATGCATGACAGAGCCC |
| Hur1 | AGCATCAACCTCCGACCAAGTGCGGGTGTA |
| Hur2 | ATCCGGTGCAGGGGGGGGTAGAGGGGCCGC |
| For sequencing of the HVR region from viruses recovered from pigs and chickens | |
| Av N1 | TTACCATTGACTTTGAACGGCG |
| Av N3 | GCTTGTGCATTGACGATTTCCC |
| Sw N1 | CAGGTCAGGATTTCATCTAATGG |
| Av N2 | CCGGGCTGATGGTCTCGATTAG |
| Av N4 | CAATAGGTTACCCACGATGACG |
| Sw N2 | GCCCTCACGCATAATGAACTCAG |