TABLE 2.
Detection of avian HEV RNAa
| Group | Chicken no. | Result (fecal/serum) at week p.i.:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | ||
| A (aHVRd1) | 5357 | −/− | −/− | −/− | −/− | −/− | −/− | +/− | −/− | −/− |
| 5361 | −/− | −/− | −/− | +/− | −/− | −/− | +/− | −/− | −/− | |
| 5368 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |
| B (aHVRd2) | 5353 | −/− | −/− | −/− | +/+ | −/− | −/− | −/− | −/− | +/− |
| 5372 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |
| 5388 | −/− | −/− | +/− | +/+ | −/+ | −/+ | −/+ | −/− | −/− | |
| C (aHVRd3) | 5354 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 5355 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |
| 5385 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |
| D (wild-type clone) (positive control group) | 5362 | −/− | −/− | +/− | +/+ | −/− | −/− | −/− | −/− | −/− |
| 5367 | −/− | −/− | − | −/− | +/− | −/− | −/− | −/− | −/− | |
| 5374 | −/− | −/− | −/+ | +/+ | −/− | −/− | −/− | −/− | −/− | |
| E (negative control group) | 5352 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− |
| 5358 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |
| 5369 | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | −/− | |
a
Positive (+) or negative (−) samples at the indicated week p.i. in SPF chickens intrahepatically inoculated with capped RNA transcripts of avian HEV HVR deletion mutants, as well as the wild-type avian HEV infectious clone.