FIG. 3.

Characterization of SL3-3 wt- and 3mGR-induced tumors by three-color flow cytometry. Shown are dot plots of data obtained from two different tumor samples from SL3-3 wt-injected (columns A and B) and SL3-3 3mGR-injected (columns C and D) mice with the more frequent (17/23 and 27/30, respectively) (columns A and C) and less frequent (3/23 and 2/30, respectively) phenotypes (columns B and D) stained with fluorochrome-conjugated antibodies to CD3/CD11b, CD3/B220, CD4/CD8a, and TCR/HSA. The region used for the gating of the cells, as defined by forward (FSC) and side (SSC) scatter properties, is shown in all cases. The percentages of cells within each quadrant are indicated in the upper right corner. All CD4-, CD8a-, HAS-, and TCR-positive cells were CD3⁺.