FIG. 1.
(Top) Physical map of the HSV-1 genome showing the location of the UL25 gene. UL and US refer to the long and short unique region sequences, respectively, and TRL and TRS and IRL and IRS refer to the terminal and internal repeat sequences, respectively. (Middle) Schematic representation of the 580-amino-acid UL25 protein. Dashed regions are the folded protein core as determined by characterization of the UL25 crystal structure (5). Numbers above the line indicate the amino acid location where 15 bp were inserted into the UL25 ORF, resulting in either the insertion (i) of five amino acids or an in-frame stop codon (s). (Bottom) The locations of the insertions and the predicted amino acid changes are shown along with the predicted molecular mass (MM) for each mutant. *, stop codon.