FIG. 1.
PT and HK dramatically reduce picornavirus multiplication and increase cell viability. Viral titers of HRV2-infected HeLa cells (MOI = 20) in the presence or absence of indicated concentrations of PT (A) or HK plus 30 mM MgCl2 (B) were determined 24 h p.i. Results for one representative experiment of three experiments are shown. HeLa cells were infected with HRV2 (MOI = 50) or mock infected, and different concentrations of PT (C) or HK-MgCl2 (D) were added. At 24 h p.i., a CellTiter 96 AQueous nonradioactive cell proliferation assay was performed according to the manufacturer's protocol. Extinction at 492 nm reflects cell viability. The means ± standard deviations of quintuples obtained in one representative experiment of three performed are shown. (E) HeLa cells were infected with CVB3 or mengovirus (MOI = 10) and incubated with 10 μM PT or 75 μM HK-30 mM MgCl2, respectively. Virus production of infected cells was determined by TCID50 assay at 24 h p.i. The results for one of two representative experiments are shown. −, control.