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Substitutions improve the folding and secretion of stabilized gp140 constructs. The ΔV1/V2.6 deletion and compensatory changes identified in various evolution cultures were introduced in an expression vector for SOS gp140. The variants were expressed in 293T cells, and intracellular and secreted Env was analyzed by SDS-PAGE, BN-PAGE, and Western blotting. d, day.
