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. 2008 Oct 22;83(1):273–282. doi: 10.1128/JVI.01532-08

FIG. 1.

FIG. 1.

Deletion of the EG-EA repeat from HVS ORF73 leads to an increase in the total amount of ORF73 expressed. (A) Schematic representation of the HVS ORF73 constructs used to investigate the effects of the EG-EA repeat on protein expression. 73-GFP is full-length ORF73, with the EG-EA acidic domain highlighted, tagged with GFP at the C terminus; 73Δ-GFP is ORF73 with the EG-EA repeat deleted and again tagged with GFP; 73-E3A-GFP contains a 200-amino-acid fragment derived from EBV EBNA3A in place of the EG-EA repeat. (B) Immunoblot analysis of expression of 73-GFP, 73Δ-GFP, and 73-E3A-GFP. 293 cells were transfected with the indicated plasmids, and total protein was extracted at 48 h posttransfection. Twenty μg of protein was resolved on 10% SDS-PAGE gels and transferred to nitrocellulose. Duplicate blots were probed with either anti-GFP, to detect the ORF73 constructs, or anti-actin as a loading control.