TABLE 2.
Reactive peptides in Nef pools 2 to 5, magnitudes of response, frequencies, and known epitopes within the sequence
| Reactive-peptide sequence | % Responders (no./total) | Mean response ± SD (SFU/106 PBMC) | Range (SFU/106 PBMC) | Described epitopesa |
|---|---|---|---|---|
| EVGFPVRPQVPLRPMTYKA | 16 (3/9) | 554 ± 214 | 390-960 | FPVRPQVPL (B7; B3501); RPQQVPLRPM (B4201; B8101); PQVPLRPMTY (B35); QVPLRPMYTK (A3; A11); VPLRPMTY (B35; B42); PLRPMTYK (A11) |
| RPMTYKAAFDLSFFLKEKG | 36 (9/25) | 382 ± 320 | 110-1,255 | KAAFDLSFF (B5701; B5801); AAFDLSFFL (B5703); DLSFLKEK (A3; A11) |
| IHSKRRQDILDLWVYYHTQG | 24 (6/25) | 1,240 ± 1,113 | 150-3,915 | RQDILDLWV (B1302); RQDILDLWVY (Cw07; B7); ILDLWVYHT (A2) |
| WVYHTQGYFPDWQNYTPGP | 12 (3/25) | 1,567 ± 1,578 | 145-3,710 | YHTQGYFPDW (B17); HTQGYFPDW (B57; B5801); TQGYFPDWQNY (B15); GYFPDWQNY (A24); YFPDWQNY (A29; B57; B5801); YFPDWQNYT (A01) |
| PGPGVRYPLTFGWCFKLVP | 80 (8/10) | 2,005 ± 1,503 | 170-4,800 | TPGPGVRYPL (B7;) GPGVRYPLTF (B35); VRYPLTFGW (B27); RYPLTFGW (A2301; A24); YPLTFGWCF (B18; B35; B5301); LTFGWCFKL (A2) |
| NNCLLHPMSQHGMEDADRE | 14 (1/7) | 570 ± 169 | No previously defined epitopes |
a
From reference 23.