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Disruption of CoREST-ICP0 interaction by the D671A/E673A mutation. HeLa cells were mock infected or exposed to 10 PFU of HSV-1(vCPc0), the RF mutant, R8507, or R8508 per cell for 7 h. Immunoprecipitation (IP) was carried out as described in Materials and Methods. The immunoprecipitates were immunoblotted with CoREST or ICP0 antibody. The input was 5% of the total lysates used for immunoprecipitation.
