FIG. 7.

Protein content of virions and L-particles. Extracellular virions and L-particles from cells infected with HSV-1 strain 17⁺ (WT) and L-particles from cells infected with mutant virus K5ΔZ, FRΔUL37, KΔUL36, or ARΔUL36 were separated by Ficoll gradient sedimentation (see Fig. S4 in the supplemental material). The gradients were collected from the bottom in 10 equal fractions. A control sample of HSV-1 strain 17⁺ L-particles (LP) was run on each gel. Gradient fractions were resolved by SDS-PAGE and analyzed by Western blotting. Blots were probed sequentially with E12-E3 (anti-pUL36) (B), M780 (anti-pUL37) (C), and AGV031 (anti-pUL49) (A). The position of the 43-kDa N-terminal fragment of pUL36 is indicated in panel B.