FIG. 4.

Cryo-FESEM images showing the inner (i) and parts of the outer (o) nuclear membranes of HSV-1-infected HeLa cells at 10 h of incubation. (A) Nuclear pores appear as button-like structures or holes. There are also slightly larger holes with distinct borders and small protrusions of similar size (arrows) as well as three holes with diameters of up to 250 nm and a few budding capsids. (B) The number of budding capsids is about 1.8/μm². One budding is probably close to completion (arrowhead). There are holes from 110 to 220 nm, two protrusions (arrows) of 130 nm in diameter, and folds of the nuclear surface. Bars, 200 nm.