TABLE 3.
Contribution of anti-CD4-BS antibodies to the cross-neutralizing activity of plasmasa
| Subject (% breadth) | Isolate | Plasma | % Neutralization of flowthrough
|
IC50 (μg/ml) of eluted antibodies
|
||
|---|---|---|---|---|---|---|
| WT gp120 | BS gp120 | WT gp120 | BS gp120 | |||
| VC10042 (100) | YU2 | 100 | (-) | 100 | 1.2 | (-) |
| QH0692 | 90 | 19 | 100 | 1.9 | (-) | |
| TORNO | 92 | 15 | 90 | 6.6 | (-) | |
| JRFL | 99 | 10 | 99 | 1.5 | (-) | |
| VC10014 (79) | YU2 | 92 | (-) | 15 | 6.5 | 8.1 |
| QH0692 | 52 | 12 | 28 | 14.5 | 19.1 | |
| TORNO | 70 | 25 | 45 | 12.3 | 14.6 | |
| JRFL | 95 | 5 | 35 | 7.3 | 10.3 | |
| CC1161 (84) | YU2 | 89 | 37 | 65 | 1.4 | (-) |
| QH0692 | 73 | 44 | 62 | 6.1 | (-) | |
| TORNO | 95 | 82 | 81 | 1.8 | 3.8 | |
| JRFL | 98 | 88 | 89 | 3.9 | 1.8 | |
| VC10002 (32) | YU2 | 40 | (-) | (-) | (-) | (-) |
| QH0692 | 85 | 53 | 60 | 7.2 | 10.8 | |
| TORNO | 72 | 52 | 57 | 8.9 | 15 | |
| JRFL | 90 | 47 | 55 | 8.6 | 13.4 | |
| CC1508 (95) | YU2 | 95 | 91 | 92 | (-) | (-) |
| QH0692 | 98 | 68 | 75 | 13.6 | 14.2 | |
| TORNO | 90 | 82 | 86 | 8.1 | 13.4 | |
| JRFL | 100 | 100 | 99 | (-) | (-) | |
| VC20013 (63) | YU2 | 27 | 12 | 22 | (-) | (-) |
| QH0692 | 78 | 78 | 72 | (-) | (-) | |
| TORNO | 73 | 73 | 72 | (-) | (-) | |
| JRFL | 70 | 63 | 58 | 8.4 | 9.7 | |
| VC20011 (5) | YU2 | (-) | (-) | (-) | (-) | (-) |
| QH0692 | (-) | (-) | (-) | (-) | (-) | |
| TORNO | (-) | (-) | (-) | (-) | (-) | |
| JRFL | (-) | (-) | (-) | (-) | (-) | |
a
The anti-HIV-neutralizing activities of the flowthrough from the WT gp120 and the CD4-BS mutant gp120 (BS gp120) and those of the antibodies eluted from the WT gp120 and CD4-BS mutant gp120 were determined as discussed in Materials and Methods. For the plasma and flowthroughs, the percent neutralization at a 1:50 dilution is shown. Significant neutralization (≥50%) is shown in boldface. For the eluted antibodies, the IC50s are shown. (-), neutralizing activity (50% neutralization) against the indicated virus was not recorded at a 1:50 dilution. The overall breadth of neutralization for each plasma is shown under each code identifier.