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. 2008 Nov 12;83(2):584–597. doi: 10.1128/JVI.01443-08

FIG. 4.

FIG. 4.

Expression levels of HIV Gag, driven by the different recombinant NDVs. (A) Lysates from Vero cells infected with the NDV-HIVGag and NDV-GFP viruses were subjected to SDS-PAGE and were transferred to nitrocellulose membranes. Membranes were immunoblotted using an anti-NDV rabbit serum (α-NDV) and an anti-p24 (α-p24) human monoclonal antibody (71-31), followed by incubation with an anti-rabbit IgG peroxidase-labeled antibody and an anti-human IgG peroxidase-labeled antibody, respectively. (B) Lysates from Vero cells infected with the NDV-HIVGag and NDV-GFP viruses at 12, 24, 36, or 42 h postinfection were separated by SDS-PAGE, and Western blotting was performed using anti-NDV rabbit serum and anti-p24 mouse monoclonal antibody (24-4), followed by incubation with an anti-rabbit IgG peroxidase-labeled antibody and an anti-mouse IgG peroxidase-labeled antibody, respectively. (C) Vero cells were infected at an MOI of 1 with the indicated NDV viruses. Media from the infected cells were harvested at the indicated time points, and virus titers were measured by indirect immunofluorescence in Vero cells.