FIG. 6.

Generation of recombinant NDV containing a mammalian codon-optimized version of HIV gag. (A) A recombinant virus expressing a synthetic codon-optimized version of HIV gag (NDV-HIVGag-opt) inserted between the P/V and M genes was rescued following the same protocol described for earlier experiments. (B) Lysates from Vero cells infected with NDV-GFP, NDV-HIVGag-XbaI, or NDV-HIVGag-opt at an MOI of 1 were separated in an SDS-PAGE gel and transferred to nitrocellulose membranes. Membranes were immunoblotted using an anti-NDV rabbit serum and an anti-p24 mouse monoclonal antibody, 24-4, followed by incubation with an anti-rabbit IgG peroxidase-labeled antibody and an anti-mouse IgG peroxidase-labeled antibody, respectively.