FIG. 9.

PKC-θ overexpression recovers the normal expression levels of COX-2 and TNF-α inhibited by A238L. Vero cells were transiently cotransfected with COX-2 (A) or TNF-α (B) promoter reporter plasmids (250 ng/10⁶ cells) as indicated in the figure, together with pEF-PKC-θ(wt) or the constitutively active mutant pEF-PKC-θ(A/E) expression plasmids (1 μg/10⁶ cells). Sixteen hours after transfection, Vero cells were infected with Ba71Vwt or Ba71VΔA238L at a MOI of 5 PFU/cell. At the indicated postinfection times, whole-cell extracts were prepared and luciferase activity was assayed. Extracts were normalized to Renilla luciferase activity as described in Materials and Methods. RLU per μg of protein from triplicate transfections (means ± standard deviations) are shown.