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. 2008 Nov 5;83(2):1093–1104. doi: 10.1128/JVI.01633-08

FIG. 5.

FIG. 5.

Differential consequences of the addition of GFP or Vpr to the C-terminal end of Nef. (A) Viruses were produced in the presence of WT Nef (left) or Nef-GFP (right). Cells and viruses were analyzed as described in the Fig. 2A legend. Nef-GFP was detected with GFP-specific antibodies. (B) Viral infectivities of viruses produced in the presence of the indicated variants of Nef were analyzed as described in the Fig. 2C legend. (C) Viruses were produced in the presence of the indicated combination of Nef, Nef-Vpr, or Vpr. Cells and viruses were analyzed as described in the Fig. 2A legend. Arrowheads point out the 27- and the 18-kDa forms of Nef. (D) Viral infectivities of viruses produced in the presence of the indicated combinations of Nef, Nef-Vpr, and/or Vpr were measured as indicated in the Fig. 2C legend and normalized to that of viruses produced in the presence of Nef Xho.