FIG. 7.

Characterization of the Nef-ERQAN fusion protein. (A) Viruses were produced in the presence of the indicated combination of Nef, Nef-ERQAN, and Nef-CS-Vpr. Cells and viruses were analyzed as described in the Fig. 2A legend. The amounts of Nef-CS-Vpr-coding plasmid cotransfected with Nef and Nef-ERQAN were adjusted to achieve incorporation of similar amounts of Nef-CS-Vpr and Nef in viral particles. (B) Viral infectivities of viruses produced in the presence of the indicated combinations of Nef, Nef-ERQAN, and Nef-CS-Vpr were measured as indicated in the Fig. 2C legend and normalized to that of viruses produced in the presence of Nef Xho. * indicates a product cleaved by the viral protease.