FIG. 10.

H9 exosomes reduce accumulation of HIV-1 reverse transcripts and two-LTR circles. SupT1 cells were preincubated for 16 h with H9 or SupT1 exosomes or left untreated (control). Cells were infected for 3 h either with VSV-G-pseudotyped NL4-3-ΔE-EGFP (A to C) or with wild-type NL4-3 in the presence of protease inhibitors amprenavir and nelfinavir, each at 5 μM (D to F), washed, and cultured without (control) or with an additional dose of exosomes. Total DNA was isolated at indicated times postinfection. Relative levels of HIV-1 ERT (A and D), LRT (B and E), and two-LTR circles (C and F) were quantified by real-time PCR. Error bars, means ± SDs of triplicate samples from a representative experiment.