FIG. 5.
A3G exosomes inhibit replication of vif-negative HIV-1 in Jurkat T cells. Cells (1 × 106/ml) were preincubated for 16 h with indicated doses of H9 exosomes (A) or SupT1 exosomes (B), followed by 3 h of infection with vif-negative HIV-1 (5 RT cpm/ml). After cell washing, additional indicated doses of exosomes were added to the cell culture. Virus replication was monitored by RT activity released into the culture supernatants. Error bars, mean values ± SDs of triplicate samples.