FIG. 7.

H9 exosomes inhibit HIV-1 replication in SupT1 cells. SupT1 cells were pretreated with A3G-positive (H9 exo) or A3G-negative (SupT1 exo) exosomes or left untreated (control) and infected with vif-negative (ΔVif HIV-1) or wild-type NL4-3 (HIV-1 wt). On day 2 and day 3 postinfection, two additional doses of exosomes were added. (A) Cell lysates were prepared on day 5 postinfection and analyzed by Western blotting for the expression of HIV-1 Gag p24, Vif, and β-actin. (B) Replication of wild-type HIV-1 in untreated or exosome-treated cells was monitored by RT activity released into the culture supernatants.