FIG. 2.

Autoradiogram of an agarose gel with [³H]uracil-labeled infected cells of LM2489. Infection was with Φ6 or Φ8. In vitro columns show products of transcription reactions of inner cores of purified virions. Note that, in vitro, Φ6 shows very little L transcript but that Φ8 has abundant L transcript. Also note that late transcripts in Φ6-infected cells show little L transcript but that the GG mutant has a significant amount. In the case of Φ8-infected cells, there is also little L transcript unless gene H is missing (deletion of positions 329 to 1152). wt, wild type; u, uninfected cells; e, early labeling (15 min); late, labeling at 60, 90, 120, 165, and 210 min. ΔH, m8, and mJ refer to phage Φ2806. m8, mutation in gene 8; mJ, mutation in gene J.