FIG. 7.
Solution competition SPR analysis with soluble Ad11-R279Q and Ad7-Q279R knobs. Shown are solution competition analyses of Ad11-R279Q (A) and Ad7-Q279R (B) knobs competing with surface-immobilized Ad35 knob for binding to CD46. Unbound CD46-D2 was plotted against the total concentration of soluble-knob protomers (colored squares) and fitted to a “solution affinity” model. The gray shaded boxes show the determined solution KD values of the CD46-D2-soluble-knob interaction. The plots were obtained as described in the legend to Fig. 3.