TABLE 2.

Affinity data determined by SPR

Protein interaction ligand/analyte	Equilibrium analysis (KD [M])	Kinetic analysis			Solution competition analysis (KD [M])
		KD (M)	ka (M−1 s−1)	kd (s−1)
Ad35/CD46-D2	17 × 10−9	17 × 10−9	1.5 × 106	0.025	21 × 10−9
Ad35/CD46-D4	16 × 10−9	16 × 10−9	1.1 × 106	0.017	27 × 10−9
Ad11/CD46-D2	ND	11 × 10−9	1.3 × 106	0.014	23 × 10−9
Ad11/CD46-D4	ND	13 × 10−9	0.8 × 106	0.009	19 × 10−9
Ad7/CD46-D2	35 × 10−6	−a	−	−	58 × 10−6
Ad7/CD46-D4	30 × 10−6	−	−	−	65 × 10−6
Ad14/CD46-D2	27 × 10−6	−	−	−	73 × 10−6
Ad14/CD46-D4	31 × 10−6	−	−	−	63 × 10−6
Ad7-279R/CD46-D2	0.5 × 10−6	0.5 × 10−6	1.3 × 106	0.62	0.1 × 10−6
Ad11-R279Q/CD46-D2	1.9 × 10−6	1.9 × 10−6	3.9 × 105	0.75	0.8 × 10−6

^a−, on and off rates were outside the measurable range for reliable quantitative evaluation of rate constants.