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. 2008 Nov 12;83(2):981–992. doi: 10.1128/JVI.01801-08

FIG. 6.

FIG. 6.

Regulation of the viral promoter. (A) HEK-293 cells were transfected with the proviral clone pMtat(−), pEGFP-N1 (normalizing control), and the indicated expression vector in the presence (+) or absence (−) of the Tat-expressing pCMVtat vector. Total viral mRNA data were normalized for the EGFP mRNA content of each sample. (B) The construct pLTR-Luc, expressing the firefly luciferase gene under the control of the LTR promoter, was cotransfected with pEGFP-N1 and the indicated expression vector in the presence (+) or absence (−) of the pCMVtat vector. The luciferase mRNA data were normalized for the EGFP mRNA content in each sample. (C) The pNL4-3 proviral clone was cotransfected with increasing amounts of either the pA1 or pSF2 expression plasmid. Total viral mRNA amounts were normalized for the EGFP mRNA content of each sample. (D) The panels show the amount of protein present in the cells transfected with the increasing amounts of expression vectors in comparison with the cells transfected with the control pLuc vector.