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. 2008 Nov 12;83(2):981–992. doi: 10.1128/JVI.01801-08

FIG. 7.

FIG. 7.

Cellular localization of the viral mRNA. HEK 293 cells were cotransfected with the proviral clone pNL4-3, pEGFP-N1 (normalizing control) and either one of the expression vectors, or treated with siRNA. Nuclear and cytoplasmic RNA fractions were analyzed by qPCR with primers sets specific for the Gag/Pol mRNA (A), the Env1 mRNA (B), and a region specific for all the 2-kb mRNA species (C) (see primers P11 and P12 in Fig. 1). EGFP mRNA was utilized to normalize the data. Each graph summarizes the ratio of each mRNA contained in the nuclear (Nucl) versus the cytoplasmic (Cyto) fraction (labeled on the y axis) after overexpression or siRNA treatment of the different cellular factors (labeled on the x axis). The control (Cont) refers to the pLuc plasmid and the control siRNA.