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. 2008 Nov 12;83(2):981–992. doi: 10.1128/JVI.01801-08

FIG. 8.

FIG. 8.

Virion production and infectivity following expression or downregulation of hnRNPs and SR proteins. HEK 293 cells were cotransfected with the proviral clone pNL4-3 and either cotransfected with one of the expression vectors or treated with siRNA. (A) Cell viability was measured by quantifying cellular ATP production. Viability of the cells transfected with the expression clones is relative to that transfected with the pLuc control. Viability of the siRNA-treated cells is relative to that of the control siRNA. (B) Virion production was measured by quantifying the p24 viral capsid protein present in the supernatant of each transfection 72 h posttransfection by ELISA. (C) Viral infectivity assay. Cultures of TZM-bl cells were infected with equal amounts of viral particles (10 pg) as determined by p24 ELISA, derived from the supernatant harvested from the HEK 293 cells 72 h after transfection. Luciferase activity was measured in the indicator cells 72 h after infection. One hundred percent infectivity was defined as luciferase activity obtained by incubating the indicator cell line with the supernatant from the HEK 293 cells either transfected with the control pLuc plasmid or treated with the control siRNA.